The protocol combines age-specific isolation of neural stem cell-derived spontaneous mixed cultures containing differentiating oligodendrocyte precursor cells and astrocytes, assays mimicking pathological conditions, and robust, high-content reado
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We describe the production of mixed cultures of astrocytes and oligodendrocyte precursor cells derived from fetal or adult neural stem cells differentiating into mature oligodendrocytes, and in vitro modeling of noxious stimuli. The coupling with a cell-based high-content screening technique builds a reliable and robust drug screening system.