This practical can help us study dynamic changes of the nano structural components of the mechanical electrical transduction machinery at the surface of the hair cell stereocilia bundles. The main advantage of this technique is the time-lapse imag
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Here we present a protocol for the Hopping Probe Ion Conductance Microscopy (HPICM), a non-contact scanning probe technique that allows nanoscale imaging of stereocilia bundles in live auditory hair cells.