Harvest and Preparation of Intervertebral Disc (IVD) Samples
2:11
Tissue Fixation and Histologic Sectioning
3:00
Microscopic Imaging and Processing
4:11
Cellular Pattern Identification and Density Assessment
4:29
Results: Microscopic Analysis of IVDs Through Various Developmental Stages
5:45
Conclusion
Transcrição
Intervertebral Disc degeneration leads to a high degree of impairment and pain. Our protocol allows us to investigate morphologically disc development and its changes in degeneration. The dense collagen type one network makes it usually difficult
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We present a method to investigate spatial chondrocyte organization in the anulus fibrosus of the intervertebral disc using an optical sectioning method.