September 16th, 2022
•A 96-well microtiter plate-based protocol using a 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-carboxanilide-2H-tetrazolium (XTT) reduction assay is described herein, to study antibodies' effects on biofilms formed by C. tropicalis. This in vitro protocol can be used to check the effect of potential new antifungal compounds on the metabolic activity of Candida species cells in biofilms.
Tags
Vídeos Relacionados
Co-culture Models of Pseudomonas aeruginosa Biofilms Grown on Live Human Airway Cells
A 96 Well Microtiter Plate-based Method for Monitoring Formation and Antifungal Susceptibility Testing of Candida albicans Biofilms
A Cell Free Assay System Estimating the Neutralizing Capacity of GM-CSF Antibody using Recombinant Soluble GM-CSF Receptor
The Synergistic Effect of Visible Light and Gentamycin on Pseudomona aeruginosa Microorganisms
Using Click Chemistry to Measure the Effect of Viral Infection on Host-Cell RNA Synthesis
In Vitro Assay to Evaluate the Impact of Immunoregulatory Pathways on HIV-specific CD4 T Cell Effector Function
Use of a Monocyte Monolayer Assay to Evaluate Fcγ Receptor-mediated Phagocytosis
A High-throughput Compatible Assay to Evaluate Drug Efficacy against Macrophage Passaged Mycobacterium tuberculosis
Fluorescence-based Neuraminidase Inhibition Assay to Assess the Susceptibility of Influenza Viruses to The Neuraminidase Inhibitor Class of Antivirals
A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados