Preparation of ConA‐Sepharose Beads and ConA‐Sepharose: Amylopectin Beads
2:19
Starch Excess4 (SEX4) Protein Binding with ConA‐Sepharose: Amylopectin Beads
3:41
Running SDS‐PAGE Gels
4:27
Western Blotting for Chemiluminescence Detection and Data Analysis
6:28
Results: Binding Affinity of SEX4 to ConA‐Sepharose‐Amylopectin Beads
8:18
Conclusion
TRANSCRIÇÃO
This concanavalin A-based in vitro sedimentation assay is designed to measure binding affinity of glucan phosphatases against different STA substrates. The information we gain from this method is fundamental to our understanding of glucan phosphat
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This method describes a lectin-based in vitro sedimentation assay to quantify the binding affinity of glucan phosphatase and amylopectin. This co-sedimentation assay is reliable for measuring glucan phosphatase substrate binding and can be applied to various solubilized glucan substrates.