October 16th, 2008
•This is intended as an introduction to patch clamp recording from Xenopus laevis oocytes. It covers vitelline membrane removal, formation of a gigaohm seal (gigaseal), and the optional conversion of the patch to the outside-out topology.
Vídeos Relacionados
Methods for Patch Clamp Capacitance Recordings from the Calyx
Pressure-polishing Pipettes for Improved Patch-clamp Recording
Microinjection of Xenopus Laevis Oocytes
Visualizing RNA Localization in Xenopus Oocytes
Fertilization of Xenopus oocytes using the Host Transfer Method
Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells
Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes
Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells
Giant Liposome Preparation for Imaging and Patch-Clamp Electrophysiology
The Xenopus Oocyte Cut-open Vaseline Gap Voltage-clamp Technique With Fluorometry
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados