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Abstract

Environment

Measuring Enzymatic Stability by Isothermal Titration Calorimetry

Published: March 26th, 2019

DOI:

10.3791/59302

1Department of Nutrition, Dietetics and Food Science, Brigham Young University, 2Department of Chemistry and Biochemistry, Brigham Young University

Abstract

This work demonstrates a new method for measuring the stability of enzyme activity by isothermal titration calorimetry (ITC). The peak heat rate observed after a single injection of the substrate solution into an enzyme solution is correlated with enzyme activity. Multiple injections of the substrate into the same enzyme solution over time show the loss of enzyme activity. The assay is autonomous, requiring very little personnel time, and is applicable to most media and enzymes.   

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Keywords Enzyme Stability

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