Generation of Ventricular-Like HiPSC-Derived Cardiomyocytes and High-Quality Cell Preparations for Calcium Handling Characterization

Summary

Here we describe and validate a method to consistently generate robust human induced pluripotent stem cell-derived cardiomyocytes and characterize their function. These techniques may help in developing mechanistic insight into signaling pathways, provide a platform for large-scale drug screening, and reliably model cardiac diseases.

Abstract

Human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) provide a valuable human source for studying the basic science of calcium (Ca²⁺) handling and signaling pathways as well as high-throughput drug screening and toxicity assays. Herein, we provide a detailed description of the methodologies used to generate high-quality iPSC-CMs that can consistently reproduce molecular and functional characteristics across different cell lines. Additionally, a method is described to reliably assess their functional characterization through the evaluation of Ca²⁺ handling properties. Low oxygen (O₂) conditions, lactate selection, and prolonged time in culture produce high-purity and high-quality ventricular-like cardiomyocytes. Similar to isolated adult rat cardiomyocytes (ARCMs), 3-month-old iPSC-CMs exhibit higher Ca²⁺ amplitude, faster rate of Ca²⁺ reuptake (decay-tau), and a positive lusitropic response to β-adrenergic stimulation compared to day 30 iPSC-CMs. The strategy is technically simple, cost-effective, and reproducible. It provides a robust platform to model cardiac disease and for the large-scale drug screening to target Ca²⁺ handling proteins.

Introduction

Human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) are an attractive human-based platform to model a large variety of cardiac diseases in vitro^{1,2,3,4,5,6,7,8}. Moreover, iPSC-CMs can be used for the prediction of patient responses to novel or existing drugs, to screen hit compounds, and develop new personalized drugs^9,10. However, despite significant progress, several limitations and challenges need to be considered when using iPSC-CMs¹¹. Consequently, methods to improve cardiac differentiation protocols, to enhance iPSC-CMs efficiency and maturation, and to generate specific cardiomyocyte subtypes (ventricular, atrial, and nodal) have been intensely studied and already led to numerous culture strategies to overcome these hurdles^12,13,14,15.

Notwithstanding the robustness of these protocols, a major concern for the use of iPSC-CMs is the reproducibility of long and complex procedures to obtain high-quality cardiomyocytes that can ensure the same performance and reproducible results. Reproducibility is critical not only when comparing cell lines with different genetic backgrounds, but also when repeating cellular and molecular comparisons of the same cell line. Cell variability, such as well-to-well differences in iPSCs density, may affect cardiac differentiation, generating a low yield and poor-quality cardiomyocytes. These cells could still be used to perform experiments that do not require a pure population of CMs (e.g., when performing Ca²⁺ transient measurements). Indeed, when performing electrophysiological analysis, the non-CMs will not beat, neither spontaneously nor under electrical stimulation, so it will be easy to exclude them from the analysis. However, because of the poor quality, iPSC-CMs can show altered electrophysiological characteristics (e.g., irregular Ca²⁺ transient, low Ca²⁺ amplitude) which are not due to their genetic makeup. Therefore, especially when using iPSC-CMs to model cardiac disease, it is important not to confuse results from a poor-quality CM with the disease phenotype. Careful screening and exclusion processes are required prior to proceeding to electrophysiological studies.

This method includes optimized protocols to generate high-purity and high-quality cardiomyocytes and to assess their function by performing Ca²⁺ transient measurements using a calcium and contractility acquisition and analysis system. This technique is a simple, yet powerful, way to distinguish between high efficiency and low efficiency iPSC-CM preparations and provide a more physiologically relevant characterization of human iPSC-CMs.

Protocol

The experiments using adult rat cardiomyocytes in this study were conducted with approved Institutional Animal Care and Use Committee (IACUC) protocols of Icahn School of Medicine at Mount Sinai. The adult rat cardiomyocytes were isolated from Sprague Dawley rat hearts by the Langendorff-based method as previously described¹⁶.

1. Preparation of Media

1. Prepare hiPSC media.
   1. Equilibrate the supplement and the basal medium to room temperature (RT). Ensure that the supplement has thawed completely. Mix 400 mL of the basal medium and 100 mL of the supplement and filter using a 0.22 μm vacuum-driven filter. Store at 4 °C and equilibrate to RT before use.
2. Prepare RPMI + B27.
   1. Equilibrate the B27 supplement and the basal medium (RPMI 1640) to RT. Ensure that the supplement has thawed completely. Mix 490 mL of the basal medium and 10 mL of the 50x supplement and filter using a 0.22 μm vacuum driven filter. Store at 4 °C and equilibrate to RT before use.
3. Prepare RPMI + B27 (-) insulin.
   1. Equilibrate the B27 (-) insulin supplement and the basal medium (RPMI 1640) to RT. Ensure that the supplement has thawed completely. Mix 490 mL of the basal medium and 10 mL of the 50x supplement and filter using a 0.22 μm vacuum driven filter. Store at 4 °C and equilibrate to RT before use.
4. Prepare selection media (RPMI (-) glucose + B27 + lactate).
   1. Equilibrate the B27 supplement and the basal medium (RPMI 1640 (-) glucose) to RT. Ensure that the supplement has thawed completely. Mix 490 mL of the basal medium and 10 mL of the 50x supplement, add 4 mM sodium lactate constituted in sterile water, and filter using a 0.22 μm vacuum driven filter. Store at 4 °C and equilibrate to RT before use.
5. Prepare RPMI 20.
   1. Equilibrate the basal medium (RPMI 1640) to RT. Mix fetal bovine serum (FBS) (20% final concentration) and RPMI. Filter using a 0.22 μm vacuum driven filter and store at 4 °C. Equilibrate to RT before use.
6. Prepare passaging media by adding Rho-associated, coiled-coil containing protein kinase (ROCK) inhibitor (2 μM final concentration) to hiPSC media.
7. Prepare D0 media by adding GSK-3 inhibitor, CHIR 99021 to RPMI + B27 (-) insulin media (10 μM final).
8. Prepare D3 and D4 media by mixing RPMI + B27 (-) insulin media with IWR-1 (5 μM final).
   NOTE: D1 and D5 media is constituted of RPMI + B27 (-) insulin. D7 media is constituted of RPMI + B27.
9. Prepare blocking buffer (2% bovine serum albumin [BSA], 2% FBS, 0.05% NP-40 in phosphate-buffered saline [PBS]): In a 50 mL conical tube, add 1 g of BSA, 1 mL of FBS, 49 mL of PBS, and 250 μL of NP-40. Mix until fully dissolved.

2. Preparation of Human Embryonic Stem Cell (hESC)-qualified Matrix Coated Plates and Coverslips

NOTE: Perform all the steps under a sterilized tissue culture hood.

1. Thaw hESC-qualified matrix stock solution overnight on ice at 4 °C. Refer to the product specification sheet to determine appropriate aliquot volumes as this may vary depending upon the stock. Store these aliquots at -20 °C in 1.5 mL microcentrifuge tubes.
2. In order to use the matrix for coating plates or glass coverslips, first thaw an aliquot of hESC-qualified matrix at 4 °C for 30 min.
3. Aliquot 24 mL of cold Dulbecco's modified Eagle medium (DMEM): nutrient mixture F-12 (DMEM/F:12) media into a 50 mL conical tube.
4. Mix the cold DMEM/F:12 media with a 2 mL glass pipette in order to cool down the surface of the pipette.
5. Using the same pipette, take up approximately 500−700 μL of cold DMEM/F:12 and mix with the hESC-qualified matrix aliquot within the microcentrifuge tube itself.
6. Once properly mixed, transfer the solution to the 50 mL conical tube containing the cold DMEM/F:12 and mix again.
7. For a standard 6 well plate, add 1 mL of this mixture to each well. Ensure that the well is entirely covered. Leave the plates at RT underneath the tissue culture hood for at least 30 min. If desired, store at 4 °C immediately after plating for up to 1 week and equilibrate to RT for 30 min prior to use.
8. In order to use the plates, aspirate the matrix and replace with appropriate media. Use immediately.
9. Store the glass coverslips in a sterile environment (e.g., inside a sterile tissue culture hood).
10. Before coating, wipe each individual coverslip with 70% ethanol. Once the coverslip is dry, place it inside a well of a sterile 6 well plate.
11. Take 250−300 μL of the hESC-qualified matrix solution and carefully dispense it directly onto the center of the glass coverslip. Leave the coverslips at RT underneath the tissue culture hood for at least 30 min before use.

3. Preparation of Small Molecules

NOTE: Reconstitute all small molecules and Wnt modulators in DMSO unless otherwise stated.

1. Prepare 10 mM aliquots of 25 µL each of IWR-1 and CHIR 99021 and store at -20 °C.
2. Reconstitute 10 mM aliquots of 50 µL each of Thiazovivin (ROCK inhibitor) and store at -20 °C.

4. Maintenance and Passaging of iPSCs

NOTE: Perform all of the following steps under a sterile tissue culture hood.

1. Maintain the iPSCs in standard 6 well plates and perform all steps under sterile conditions. Maintain the cells with 2 mL of hiPSC media per well. Change the media every other day. Keep the cells at 37 °C, 6% O₂, 5% CO₂. Passage the cells when they are between 70−80% confluent.
2. Equilibrate PBS without Ca²⁺ and Mg²⁺ to RT.
3. To start the passaging process, add 1 mL of PBS without Ca²⁺ and Mg²⁺ to the well that needs to be passaged. Incubate at RT for 7−10 min. Check the cells underneath the microscope to ensure that PBS treatment has not resulted in complete dissociation of the monolayer.
4. Remove PBS and replace with 1 mL of passaging media. Use a cell lifter to gently scrape and lift the cells from the surface of the well.
5. Mechanically dissociate the cells using a sterile 2 mL glass pipette. Repeat until the cells are well dissociated evenly into small colonies when observed under a microscope.
6. Once the cells have been sufficiently dissociated, add 5 mL of passaging media to split the cells 1:6. Adjust the amount of passaging media to be added to match the preferred split ratio.
7. Aspirate the hESC-qualified matrix from the hESC-qualified matrix-coated plate and replace with 1 mL of passaging media per well. Add 1 mL of dissociated cells per well.

5. Cardiomyocyte Differentiation

1. Use hiPSC lines that are well-established (more than 20 passages) and exhibit a homogeneous morphology before starting cardiac differentiation.
2. Ensure that the iPSCs are around 70−80% confluent.
3. Wash the cells 1x in PBS without Ca²⁺ and Mg²⁺.
4. Add 2 mL of D0 media (step 1.7) per well and transfer the cells back to the incubator.
5. After 24 h, replace with 3 mL of D1 media per well for 48 h.
6. On day 3, replace the media with 2 mL of D3 media per well. Repeat with D4 media on day 4.
7. On day 5, replace the media with 3 mL of D5 media per well.
8. On day 7, replace the media with 3 mL of D7 media per well and transfer to an incubator with 37 °C, 5% CO₂, and normal O₂ concentration. Replace the D7 (RPMI + B27) media every 2 days.

6. Selection Procedure and iPSC-CM Dissociation

1. Ten days before performing the Ca²⁺ transient measurements or any functional analysis, replace the RPMI + B27 media with 3 mL of selection media per well for 48 h.
2. Replace the media with 3 mL of selection media for another 48 h.
3. Replace the media with 2 mL of RPMI + B27 media per well for 24 h.
4. Coat standard 6 well plates as described in section 2.
5. Add 1 mL of sterile 0.25% trypsin with EDTA to each well. Incubate the plate at 37 °C for 5 min.
6. Using a 1,000 μL pipette, mechanically dissociate the cells so that single cells can be seen when observed under a microscope.
7. Transfer the cells to a sterile 15 mL conical tube and add 2 mL of RPMI 20 media per well. Centrifuge for 5 min at 800 x g.
8. Aspirate the supernatant and resuspend the cells in RPMI + B27 media. Aspirate the hESC-qualified matrix from the plates and replace with 1 mL of RPMI + B27 media.
9. Using a 1,000 mL pipette tip, mechanically dissociate the cell pellet until the solution appears homogeneous.
10. Transfer roughly 500,000 cells to each well. Transfer to incubator for 24 h.
11. Replace the media with 3 mL of selection media per well for 48 h.
12. Replace the media with 3 mL of RPMI + B27 per well. Maintain cells in D7 media (RPMI + B2 changing media every 2 days until ready for functional analysis.

7. Preparation of iPSC-CMs for Flow Cytometry

1. Once the cells are of the desired age and have undergone metabolic selection (section 6), wash the cells with PBS without Ca²⁺ and Mg²⁺.
2. Add 1 mL per well of trypsin 0.25% and incubate for 5−7 min at 37 °C.
3. Pipette the mixture 5−10x with a P1000 tip to singularize the cells, and transfer to a 15 mL tube containing 2 mL of RPMI 20.
4. Spin the cells at 600 x g for 5 min.
5. Add 100 µL of fixation solution (4% PFA) to the cell pellet. Add the solution dropwise with continuous, gentle vortexing and then set on ice for 15 min.
6. Add 1.5 mL of PBS. Collect the cells by centrifugation and aspirate the supernatant.
7. For every experiment, include one unstained control per fixation/permeabilization condition.
8. Resuspend cells in 500 µL of blocking solution (2% FBS/2% BSA in PBS with 0.1% NP-40) for 30 min at RT.
9. Without removing the blocking solution, add the primary antibody MLC2V/MLC2A (5 mg/mL), and incubate 45 min at RT.
10. Wash with blocking buffer. Collect cells by centrifugation and aspirate solution.
11. Add secondary antibody Alexa Fluor 555/488(1:750) diluted in the blocking buffer for 45 min at RT or overnight at 4 °C.
12. Add 1.5 mL of PBS. Collect cells by centrifugation and aspirate solution.
13. Resuspend cells in 250−300 µL of PBS. Use a P1000 pipette to disaggregate the cells.
14. Prepare round bottom tubes with a 35 µm nylon mesh cell strainer cap. Pre-wet the cell strainer with 50 µL of PBS and set the tube on ice.
15. Transfer the solution with the disaggregated cells to the round bottom tubes with the cell strainer caps. Allow the cell solution to drain naturally or tap the bottom of the tube against a flat surface, as necessary, to ensure complete drainage and collection of the cells into the tube. Make sure to set the tube back on ice as soon as possible.
16. Rinse the cell strainer with 250 µL of PBS to recover any residual cells.
17. Maintain the tubes on ice and cover with aluminum foil until flow cytometry analysis.

8. Plating Cardiomyocytes onto Glass Coverslips

NOTE: Perform all steps in a sterile environment.

1. Prepare the glass coverslips as described in steps 2.10 and 2.11.
2. Once the iPSC-CMs have been selected and are of the desired age, follow steps 6.5−6.7 to dissociate the iPSC-CMs.
3. Aspirate the supernatant and resuspend the cells in a sufficient amount of RPMI 20 media to have roughly 300,000 cells per 250 μL.
4. Using a 2 mL glass pipette, mechanically dissociate the cell pellet until the solution appears homogeneous.
5. Aspirate the hESC-qualified matrix from the coverslips.
6. Using a 1000 μL pipette, mix and pull 250 μL of the solution from the 15 mL conical tube.
7. Slowly dispense the 250 μL of the solution onto the glass coverslips, taking extra care to only add to the area where the hESC-qualified matrix coating is present.
8. Transfer carefully to the incubator and leave overnight, taking care not to shake or spread the cells on the coverslips. The next morning, gently add 2 mL of D7 (RPMI + B27) media to each well with the coverslip. Change the media after 24 h and every 2 days after that.

9. Fixing Cells

1. Ensure that an adequate amount of PBS with Ca²⁺ and Mg²⁺ is equilibrated to 4 °C.
2. Prepare a 4% paraformaldehyde (PFA) solution diluted in PBS with Ca²⁺ and Mg²⁺ and equilibrate to 4 °C.
3. Once the iPSC-CMs are of appropriate age, have undergone metabolic selection (section 6), and have been plated on coverslips (section 8), wash the cells 3x with 1 mL of cold PBS with Ca²⁺ and Mg²⁺ per well.
4. Add 1 mL of cold 4% PFA and leave the cells at RT underneath the hood for 15 min.
5. Wash the cells with cold PBS to remove excess PFA.
6. Add 2 mL of PBS with Ca²⁺ and Mg²⁺ and store at 4 °C.

10. Immunofluorescence Staining

1. Remove PBS from the fixed cells and add 1 mL of blocking buffer. Incubate for 1 h at RT.
2. Remove blocking buffer and add the primary antibody (5 mg/mL) diluted in blocking buffer. Incubate overnight at 4 °C.
3. Wash 3x in PBS with Ca²⁺ and Mg²⁺ for 5 min each.
4. Add secondary antibody diluted in blocking buffer 1:1,000.
5. Cover the plate with aluminum foil to protect it from light and incubate for 45 min at RT. Keep the aluminum foil for the following steps.
6. Wash 3x in PBS with Ca²⁺ and Mg²⁺, 5 min each.
7. Add a sufficient amount of DAPI working solution to completely cover the cells and incubate at RT for 10 min.
8. Wash the sample thoroughly with PBS with Ca²⁺ and Mg²⁺ to remove excess DAPI.
9. Take new glass slides and add a drop of mounting media in the middle. Use the dropper to evenly spread the mounting media. Put the coverslips with the cells face down on the slides.
   NOTE: Cells can be stored for 30 days if protected from light.

11. Assessment of Intracellular Ca²⁺ Transients

1. Once the iPSC-CMs are at least 3 months old, have undergone metabolic selection (section 6), and have been plated on coverslips, treat them with 2 μL of Fura-2, AM (final concentration: 1 μM) and incubate at 37 °C for 10 min.
   NOTE: Fura-2 is light-sensitive. Perform all loading procedures and experiments in the dark.
2. Prepare the calcium and contractility acquisition and analysis system.
   1. Power the system ensuring that the arc lamp is initiated (Figure 1B).
   2. Place the chamber on the system and connect the tubes from the pump to the appropriate inlet and outlets and the electric wire from the stimulator to the chamber as shown in Figure 1C.
   3. Fill the perfusion tube that runs through the fluidic inline heater with 37 °C prewarmed Tyrode's solution.
   4. Adjust the camera and framing aperture dimensions to minimize background area.
3. Mount a glass coverslip with iPSC-CMs into the chamber and fasten.
4. Add 500 μL of Tyrode's solution directly on top of the fastened glass coverslip gently and start perfusing the chamber (1.5 mL/min) with Tyrode's solution.
5. Pace iPSC-CMs with 1 Hz field stimulation using the electrical stimulator (10 V, 4 ms).
6. Incubate iPSC-CMs in the chamber with stimulation for at least 3−5 min for the cells to wash the Fura-2 dye and adapt to the environment and to wash out the fluorescent dye.
7. Adjust the viewing window to the left upper area of the glass coverslip.
8. Begin recording.
9. After collecting a consistent stream of 5−10 peaks, click Pause to temporarily stop recording.
10. Ensuring that neither the focus nor dimensions of the viewing window are altered, move the microscope's stage to the adjacent area, moving toward the opposite end, and resume recording.
11. Repeat steps 11.9 and 11.10 to scan across the coverslip, initially moving to the left, then downwards in a zig-zag fashion to cover the whole coverslip area. This consists of 80−100 measurements per coverslip.
    NOTE: Restrict the total measurement time to 10 min as secondary factors cause a decrease in Ca²⁺ transient.
12. Once the Ca²⁺ transients are acquired, analyze the data with the fluorescence traces analysis software according to the manufacturer's instructions.

Results

The protocol described in Figure 1A generated highly pure cardiomyocytes that acquire a ventricular/adult-like phenotype with time in culture. As assessed by immunofluorescence staining for the atrial and ventricular myosin regulatory light chain 2 isoforms (MLC2A and MLC2V, respectively), the majority of the cells generated by this protocol were MLC2A-positive at day 30 after induction of cardiac differentiation, while MLC2V was expressed in much lower amounts at the same t...

Discussion

Critical steps for using human iPSC-CMs as experimental models are: 1) generating high-quality cardiomyocytes (CMs) that can ensure the consistent performance and reproducible results; 2) allowing the cells to mature in culture for at least 90 days to adequately assess their phenotype; 3) performing electrophysiological studies, e.g. calcium (Ca²⁺) transient measurements, to provide a physiologically relevant functional characterization of human iPSC-CMs. We developed a monolayer-based differentiation method t...

Materials

Name	Company	Catalog Number	Comments
Anti-Actin, α-Smooth Muscle antibody, Mouse monoclonal	Sigma Aldrich	A5228
Alexa Fluor 488 goat anti mouse	Invitrogen	A11001
Alexa Fluor 555 goat anti rabbit	Invitrogen	A21428
B27 Supplement	Gibco	17504-044
B27(-) insulin Supplement	Gibco	A18956-01
CHIR-99021	Selleckchem	S2924
DAPI nuclear stain	ThermoFisher	D1306
DMEM/F12 (1:1) (1X) + L- Glutamine + 15mM Hepes	Gibco	11330-032
Double Ended Cell lifter, Flat blade and J-Hook	Celltreat	229306
Falcon Multiwell Tissue Culture Plate, 6 well	Corning	353046
Fluidic inline heater	Live Cell Instrument	IL-H-10
Fura-2, AM	Invitrogen	F1221
hESC-qualified matrix	Corning	354277	Matrigel Matrix
hPSC media	Gibco	A33493-01	StemFlex Basal Medium
IWR-1	Sigma Aldrich	I0161
Live cell imaging chamber	Live Cell Instrument	EC-B25
MLC-2A, Monoclonal Mouse Antibody	Synaptic Systems	311011
Myocyte calcium and contractility system	Ionoptix	ISW-400
Myosin Light Chain 2 Antibody, Rabbit Polyclonal (MLC2V)	Proteintech	10906-1-AP
Nalgene Rapid Flow Sterile Disposable Filter units with PES Membrane	ThermoFisher	124-0045
PBS with Calcium and Magnesium	Corning	21-030-CV
PBS without Calcium and Magensium	Corning	21-031-CV
Premium Glass Cover Slips	Lab Scientific	7807
RPMI medium 1640 (-) D-glucose (1X)	Gibco	11879-020
RPMI medium 1640 (1X)	Gibco	11875-093
Sodium L-lactate	Sigma Aldrich	L7022
StemFlex Supplement	Gibco	A33492-01
Thiazovivin	Tocris	3845
Trypsin-EDTA (0.25%)	ThermoFisher	25200056
Tyrode's solution	Boston Bioproducts	BSS-355w	Adjust pH at 7.2. Add 1.2mM Calcium Chloride