Operating Transverse Aortic Constriction with Absorbable Suture to Obtain Transient Myocardial Hypertrophy

Summary

This protocol presents an improved method to obtain transient myocardial hypertrophy with absorbable suture, simulating left ventricular hypertrophy decrease after removing pressure overload. It could be valuable for the studies on myocardial hypertrophic preconditioning.

Abstract

Based on twice transverse aortic constrictions (TACs) in mice, it is proved that myocardial hypertrophic preconditioning (MHP) could attenuate cardiomyocyte hypertrophy and slow down progression to heart failure. For novices, however, the MHP model is usually quite difficult to establish because of the technical obstacles in ventilator operation, opening the chest repeatedly, and bleeding caused by debanding. To facilitate this model, to increase the surgical success rate and to reduce the incidence of bleeding, we switched to absorbable sutures for the first TAC combing with a ventilator-free technique. Using a 2-week absorbable suture, we demonstrated that this procedure could cause significant myocardial hypertrophy in 2 weeks; and 4 weeks after surgery, myocardial hypertrophy was almost completely regressed to the baseline. Using this protocol, the operators could master the MHP model easily with a lower operation mortality.

Introduction

Ischemic preconditioning is a phenomenon that induces brief non-lethal episodes of ischemia and reperfusion to the heart and has the capacity to dramatically reduce myocardial injury¹. Given the obvious clinical implications of ischemic preconditioning, such as limiting myocardial infarct size² and suppressing ventricular tachyarrhythmias after myocardial revascularization³, there have been lots of research to dissect the mechanisms underlying cardio-protective effects induced by preconditioning^4,5. In contrast, other non-ischemic types of preconditioning have received relatively little attention. Cardiac hypertrophy may be blunted in patients with aortic stenosis undergoing aortic valve replacement⁶. Wherever the state of pathological myocardial hypertrophy exists, the principle of preconditioning is rarely reported.

In 1991, Rockman et al. firstly established a mouse model of left ventricular hypertrophy by transverse aortic constriction (TAC)⁷. By operating TAC twice in mice, we have previously proved that myocardial hypertrophic preconditioning (MHP) leads to transient hypertrophic stimulation in the heart thereby making the heart more resistant to sustained hypertrophic stress in the future⁸. The characteristics of the MHP model have been validated by ultrasound biomicroscope and hemodynamic assessment⁹. Key points in constructing the model was to perform thoracotomy three times, TAC for a week, debanding for a week, and secondary TAC for 6 weeks. However, debanding could cause bleeding, which made it difficult to be mastered by novices and difficult to be popularized. In addition, it is also a technical challenge to intubate mice. Improper intubation could cause tracheal injury, pneumothorax, and even death in mice. So, it is necessary and valuable to improve some procedures while constructing the MHP model.

To reduce the difficulty of the model and increase its success, we switched to absorbable sutures for the first TAC and monitored the model's success by measuring pressure gradient across the aortic constriction under echocardiography¹⁰. Based on our preliminary experiment, it would be difficult to induce sufficient myocardial hypertrophy in mice with too low-pressure gradient, while mice with too high-pressure gradient would develop acute heart failure or even die. The ideal pressure gradient for the model ranges from 40–80 mmHg¹¹. In addition, this experiment did not rely on a ventilator, which could effectively avoid ventilator-associated technical manipulation and injury¹².

Protocol

All procedures were carried out following the guidelines of the Care and Use of Laboratory Animals published by the US National Institutes of Health (NIH Publication No. 85-23, revised in 1996). C57BL/6J male mice (8–10 weeks, 20–25 g) were provided by the Animal Center of South Medical University.

1. Preoperative preparation

1. Pinch off the tip of a 25 G needle with a needle holder and blunt it with a hard object like the holder.
2. Pass a 5–0 absorbable suture through the needle and then curve it to 90° with a holder¹³.
   NOTE: According to different research purposes, investigators could select absorbable lines with different absorption time. In this protocol, we used a 2-week absorbable suture to constrict the aortic arch.
3. Curve another 25 G needle to 120° and smoothen the tip with a holder to be used as a spacer in the ligation step.
   NOTE: A 25 G needle was used as a spacer for mice having body weight (BW) >25 g. Use a 26 G needle for mice with 19–24 g BW.
4. Disinfect the operative site with 75% alcohol.
5. Adjust the heating pad temperature to 37 °C.
6. Prepare sterilized surgical instruments (including 1 ophthalmic scissors, 1 micro scissors, 2 microsurgical elbow tweezers, 1 needle holder, and 1 micro needle holder).

2. Induction of anesthesia and shaving

1. Anesthetize a mouse by intraperitoneal injection of a mixture of xylazine (5 mg/kg) and ketamine (100 mg/kg) diluted in saline solution (0.9% NaCl). Confirm complete anesthetization with the negative pedal withdrawal reflex.
2. Keep the mouse in supine position by fixing the incisors with a suture and fixing the limbs with adhesive tapes.
3. Apply depilatory cream to remove hair on its neck and xiphoid. Disinfect the area with iodine followed by 75% alcohol.

3. Surgery

1. Make an incision over 10 mm at the midline position between supra-sternal notch and chest with a scalpel. Then, separate the skin and the superficial fascia.
2. Identify the first intercostal space by counting the ribs from the sternal angle. Perform the incision in the first intercostal space and as close as possible to the sternum. Bluntly penetrate it with elbow tweezers to open this space.
3. Gently separate the parenchyma and the thymus until the transverse aortic arch is visible.
   NOTE: Do not damage the parietal pleura to avoid pneumothorax.
4. Pass the 5–0 absorbable suture under the aortic arch between the brachiocephalic artery and the left common carotid artery with a latch needle¹⁴. Please make sure that the brachiocephalic artery, the left common carotid artery, and the left subclavian artery are visible in the operation field.
5. Place the spacer, prepared in step 1.3, on the transverse aorta and perform a double knot on the spacer with the suture in step 3.4.
   NOTE: The tip of the spacer must be blunt to avoid damaging the transverse aorta while removing it.
6. Remove the spacer quickly but gently, and then cut the ends of the suture.
7. Close the first intercostal space and the skin using 5–0 nylon sutures. Disinfect the skin again with 75% alcohol.
8. Place the mouse on the heating pad to promote recovery. Inject buprenorphine (0.1 mg/kg, q12h) intraperitoneally for the first 3 days after the surgery.
9. Return the mouse to the cage in a 12 h light/dark cycle room when it recovers consciousness.
10. Perform sham surgery identical to all the above steps but without the constriction (step 3.5).
11. Perform surgery for the silk suture group, identical to all the above steps but using a 5–0 silk suture in step 1.2.

4. Echocardiographic assessment of successful ligation and measurements

1. Perform echocardiographic assessment on the Day (D) 7 after surgery.
2. Anesthetize the mouse with 3% isoflurane through inhalation for induction, and 1.5% for maintaining the depth of anesthesia, with a 0.5–1 L/min oxygen flow rate.
3. Place the mouse in supine position on the platform, maintained at 37 °C, and tape its limbs to the electrode.
4. Remove the chest hair with a depilatory cream and apply ultrasonic coupling agent to the mouse’s chest.
5. Assess transverse aortic constriction with a 30 MHz probe.
   1. Tilt the platform to the far left. Keep the probe in vertical position and lower it on the chest along the right parasternal line. Then, manipulate X-axis and Y-axis under B-mode until the aortic arch is clearly visible.
   2. Locate the constriction by B-mode to obtain the aortic arch view¹¹. Use the color Doppler mode and pulsed wave to measure the peak flow velocity and select mice with a velocity of more than 3,000 mm/s as the TAC group (values are based on preliminary experiments).
   3. Calculate the pressure gradient according to the modified version of Bernoulli's equation¹¹:
      pressure gradient = 4 x V_max².
      NOTE: The ideal pressure gradient for transverse aortic constriction model ranges from 40–80 mmHg¹¹.
   4. Save the data and images using Cine Store and Frame Store.
6. Assess dimensions and contractility of left ventricular (LV) with a 30 MHz probe.
   1. Reset the platform to the horizontal position. Keep the probe at 30° counterclockwise relative to the left parasternal line.
   2. Use B-mode and manipulate X and Y to obtain a clear and full-long axis view of the heart.
   3. Press M-mode to show the indicator line. Acquire images with Cine Store and Frame Store for later measurement of the LV chamber dimension, fractional shortening, and LV wall thickness.
7. Once done, stop isoflurane inhalation and allow the mouse to recover from anesthesia. Then, return the animal to its cage in a 12 h light/dark cycle room.
8. On D14 and D28 after surgery, repeat the above steps to measure the cardiac parameters and then harvest the heart for histological studies.

Results

In this study, we randomly divided 45 mice into three groups, the sham, the silk suture group, and the absorbable suture group (the number of each group on D0 (baseline), D14, and D28 after TAC was 15, 10, and 5, respectively). On D7, D14, D21, and D28 after the surgery, the constricted peak velocity was determined by echocardiography. We found that the blood flow velocity at the constriction was still greater than 3,000 mm/s in the second week after TAC even though an absorbable suture had been used to constrict the aor...

Discussion

There is still a vastly underexplored area in cardiac non-ischemic preconditioning. Based on our previous studies, we switched to using absorbable sutures to improve the myocardial hypertrophic preconditioning model.

In previous reports, many investigators used silk suture to constrict the aortic arch^8,14,15. Silk suture was easily available and was often used for surgical wound suture, tissue ligatio...

Materials

Name	Company	Catalog Number	Comments
Absorbable suture (5-0)	Shandong Kang Lida Medical Products Co., Ltd	5-0	Ligation
Animal ultrasound system VEVO2100	Visual Sonic	VEVO2100	Echocardiography
Cold light illuminator	Olympus	ILD-2	Light
Heat pad- thermostatic surgical system (ALC-HTP-S1)	SHANGHAI ALCOTT BIOTECH CO	ALC-HTP-S1	Heating
Isoflurane	RWD life science	R510-22	Inhalant anaesthesia
Matrx VIP 3000 Isofurane Vaporizer	Midmark Corporation	VIP 3000	Anesthetization
Medical nylon suture (5-0)	Ningbo Medical Needle Co.	5-0	Close the skin
Pentobarbital sodium salt	Merck	25MG	Anesthetization
Precision electronic balance	Denver Instrument	TB-114	Weighing sensor
Self-made spacer			25-gauge needle
Silk suture (5-0)	Yangzhou Yuankang Medical Devices Co., Ltd.	5-0	Ligation
Small animal microsurgery equipment	Napox	MA-65	Surgical instruments
Transmission Gel	Guang Gong pai	250ML	Echocardiography
Veet hair removal cream	Reckitt Benchiser	RQ/B 33 Type 2	Remove hair of mice
Vertical automatic electrothermal pressure steam sterilizer	Hefei Huatai Medical Equipment Co.	LX-B50L	Auto clean the surgical instruments