Direct Reprogramming of Human Fibroblasts into Myoblasts to Investigate Therapies for Neuromuscular Disorders

Summary

This protocol describes the conversion of skin fibroblasts into myoblasts and their differentiation into myotubes. The cell lines are derived from patients with neuromuscular disorders and can be used to investigate pathological mechanisms and to test therapeutic strategies.

Abstract

Investigations into both the pathophysiology and therapeutic targets in muscular dystrophies have been hampered by the limited proliferative capacity of human myoblasts. Several mouse models have been created but they either do not truly represent the human physiopathology of the disease or are not representative of the broad spectrum of mutations found in humans. The immortalization of human primary myoblasts is an alternative to this limitation; however, it is still dependent on muscle biopsies, which are invasive and not easily available. In contrast, skin biopsies are easier to obtain and less invasive to patients. Fibroblasts derived from skin biopsies can be immortalized and transdifferentiated into myoblasts, providing a source of cells with excellent myogenic potential. Here, we describe a fast and direct reprogramming method of fibroblast into a myogenic lineage. Fibroblasts are transduced with two lentiviruses: hTERT to immortalize the primary culture and a tet-inducible MYOD, which upon the addition of doxycycline, induces the conversion of fibroblasts into myoblasts and then mature myotubes, which express late differentiation markers. This quick transdifferentiation protocol represents a powerful tool to investigate pathological mechanisms and to investigate innovative gene-based or pharmacological biotherapies for neuromuscular disorders.

Introduction

Cellular models obtained directly from human tissues are useful to model many human genetic disorders, with the advantage of having the original genomic context and, in many cases, reproducing the same molecular and cellular hallmarks observed in the patients. In the field of neuromuscular disorders, muscle biopsies have been a great source of human myoblasts and have helped in the elucidation of pathological mechanisms. Additionally, they are an important tool for in vivo testing of drugs and gene therapies. On one hand, the derivation of myoblasts from muscle fragments is relatively easy. On the other hand, the culture and maintenance of primary myoblasts are challenging, because of their limited proliferation rate and replicative senescence in vitro¹. An alternative for these limitations is to immortalize myoblasts with the insertion of the human telomerase (hTERT) and/or cyclin-dependent kinase 4 (CDK4) genes^2,3, with preservation of skeletal muscle features⁴. Nevertheless, the obtention of primary myoblasts is still dependent on muscle biopsy, a surgical procedure with disadvantages to the patients, which, in many cases, have their muscles in advanced degeneration. Thus, the muscle of these patients is composed of a significant proportion of fibrotic and/or adipose tissue and yields fewer muscle cells, requiring the purification of the cells previously to the immortalization.

In contrast to muscle biopsies, skin biopsies are more accessible and are less harmful to patients. Primary fibroblasts can be derived from skin fragments in vitro. Although fibroblasts are not primarily affected by mutations causing neuromuscular disorders, they can be transdifferentiated into myoblasts. This can be achieved by the insertion of the Myod gene, a myogenic regulatory transcription factor⁵. In this manuscript, we describe the protocol to obtain transdifferentiated myoblasts, from the establishment of fibroblasts cultures to the obtention of differentiated myotubes (a representative summary of the method is depicted in Figure 1).

Pre-clinical testing of therapeutic strategies is dependent on cellular and animal models carrying mutations similar to the mutations found in human patients. Although the development of animal models has become more feasible with the advance of gene-editing technologies such as CRISPR/Cas9⁶, it is still challenging and costly. Thus, patient-derived cell lines are an accessible option to have models, covering the large spectrum of mutations of disease such as Duchenne muscular dystrophy (DMD). Obtention and creation of cell models are crucial to the development of personalized therapies for such pathologies.

Among personalized therapies that have been investigated, exon skipping strategies is one of the promising ones for different muscular dystrophies^7,8. This strategy consists of producing a shorter but functional protein. This is performed by hiding the exon definition to the spliceosome, therefore excluding the mutated exon from the final messenger. This is a very promising technology that has been approved by the FDA for DMD. Thus, we also describe in this protocol, methods to transfect myoblasts with two different exon skipping related technologies: antisense oligonucleotides (AON) and U7snRNA-adeno-associated virus (AAV). AON transfection is a good tool for the initial screening of several sequences designed to promote exon skipping⁹. However, the activity of AONs is transient. To obtain a sustained expression of antisense sequences, we also explored small nuclear RNAs (snRNAs) combined with AAV, allowing nuclear localization and inclusion in the splicing machinery¹⁰. U7 is an snRNA involved in the processing of histone mRNA that can be engineered to bind proteins that will redirect it to the spliceosome and deliver antisense sequences¹¹. The use of modified U7 snRNAs in combination with AAV vectors overcomes limitations of AONs resulting in a continued expression of the AONs and better transduction of tissues of interest¹². We use cells derived from DMD patients for this protocol to illustrate the exon-skipping strategy.

Protocol

All experiments and biopsies were carried out following the ethical rules of the institutions involved under the approval of the Nationwide Children's Hospital Institutional Review Board.

1. Initiation of dermal fibroblasts culture

1. Establishment of fibroblasts culture
   1. Aliquot 10 mL of fibroblast medium (Table 1) in 15 mL conical tubes. The skin biopsy should be placed and transported in this medium. The biopsy can be stored at 4 °C until it is processed, preferentially on the same day.
      NOTE: Use the skin biopsy within 24-36 hours to avoid potential growth of contamination.
   2. Aspirate the media from the tube and rinse the biopsy with 10 mL 1X PBS (room temperature) three times. After the third wash, leave the PBS in the tube.
   3. Pour out the PBS and the skin onto a 10 cm² dish.
   4. Using sterile scalpels, cut the biopsy into as small as possible fragments.
   5. Using a pipette, transfer an individual skin fragment and drop it into a clean 10 cm² dish. Place 10 to 12 fragments per dish.
   6. Aspirate the excess of PBS from around each fragment. Be careful to not aspirate the fragment.
   7. Cover the dishes partially with the lid and allow the skin fragments to dry for 5-20 min. Do not allow the fragments to dry excessively.
   8. Once the fragments are dry, tilt the dish at 45 degrees and slowly add 12 mL of fibroblast medium to the corner. Lower down the dish, carefully distributing the media so the fragments do not lift by the media.
   9. Place the dishes into the incubator (37 °C, 5% CO₂). Replace the media in 5-7 days, and once a week after.
   10. Observe the fibroblasts emerging from the fragment (Figure 2) and, once confluent, passage the cells into 75 cm² flasks. Remove the medium, rinse the cells with PBS and add 1 mL 0.25 % trypsin. Incubate at 37 °C for 5 min or until all cells are lifted. Add 10 mL fibroblast growth medium to inhibit the trypsin and transfer the cells to a new flask.
       ​NOTE: For passage number nomenclature, P1 is established when the first fibroblasts that emerged from the skin biopsy are transferred to a new flask for proliferation.
2. Cryopreservation of primary fibroblast lines
   1. Once the 75 cm² flask is confluent, rinse it with 10 mL 1X PBS and aspirate PBS.
   2. Add 3 mL of 0.25 % trypsin to the cell surface. Place the flask in the incubator for 5 min. Check the flasks under the microscope to see if the cells are lifted. If not, place the flask back in the incubator for an additional 5 min.
   3. Once the cells are detached, add 7 mL of fibroblast media to the flask and pipette up and down to resuspend the cells. Collect the cells into a 50 mL conical tube.
   4. Prepare 100 µL aliquot of trypan blue, and remove 100 µL from the sample being cryopreserved, mix with the trypan blue. Load the mix on the hemocytometer to count. Count the cells in four different fields of the hemocytometer under the microscope. To calculate the total number of cells, use the formula: (counted cells/100) * volume of culture.
   5. Spin the conical tubes at 300 x g for 10 minutes at room temperature or 4 °C.
   6. Aspirate off the medium and resuspend the cells in the adequate volume of freezing medium: 1 mL per each 1 million cells/vial. Pipette up and down to homogenize and distribute 1 mL to each labeled cryovial.
   7. Place the vials into the freezing box, and allow the vials to freeze at a rate of 1 °C/min at -80 °C freezer overnight.
   8. The following day transfer the vials to a liquid nitrogen tank or -150 °C freezer.

2. Establishment of FibroMyoblasts (FM) cell line

1. Seed primary fibroblasts at approximately 30% of confluency in two wells of a 12-well plate (2 x 10⁴ cells/well) in order to have about 50% of confluency the next day.
2. For lentiviral transduction, add 2 to 5 x 10⁹ vg (viral genome particles) of hTERT-puromycin lentivirus in 400 µL of fibroblast medium. To the second well, add just 400 µL of fibroblast medium. Add 1 mL of media the following day.
   NOTE: Plasmids for lentivirus production were obtained from the group that published the Chaouch et al, 2009 paper. They are also described individually in Aure et al, 2007¹³ for hTERT plasmid and Barde et al, 2006¹⁴ for the Tet-on system utilized for the design of MyoD plasmid. They were obtained thanks to a Material Transfer Agreement with Genethon, France (please contact Dr. Vincent Mouly to obtain these plasmids - vincent.mouly@upmc.fr). Briefly, the hTERT consists of hTERT variant 1 driven by a CMV promoter while the puromycin is driven by a PGK promoter. The MyoD plasmid contains a MyoD variant 1 driven by a CMV promoter under the control of the repressor rtTA2. This plasmid also contains the hygromycin selection expressed thanks to the SV40 promoter.
   Lentiviruses were produced using regular lentiviral production (see Wang and McManus JoVe protocol¹⁵). Briefly, MDL-helper, Rev-Helper, SVS-G-helper were transfected via calcium chloride precipitation also of either hTERT or MyoD plasmids. After 48 h, the supernatant was collected, and then for additional three days. All supernatant was then concentrated by ultracentrifugation. The pellet was then resuspended into Tris-HCL+NaCl+EDTA buffer. Titer estimation was evaluated by standard lentivirus qPCR assay.
3. One or two days later, transfer the cells into a 6-well plate and grow them until reaching 60-70% confluence.
4. Supplement the fibroblast medium with 1 µg/mL of puromycin and add 2 mL to each well.
5. Keep the cells under selection until all cells in the control well are dead (up to 12 days), changing media every 2-3 days. Passage the cells from the 6-well plate into two 10 cm² dishes for further proliferation.
6. Freeze vials of fibroblasts after selection. Label as F(hTer).
7. Seed hTERT-expressing fibroblasts (F(hTer)) at about 30% confluence in fibroblast medium, in two wells of a 12-well plate, to have about 50% confluence the next day.
8. For lentivirus transduction, mix 2 to 5 x 10⁹ vg of MyoD-hygromycinB lentivirus in 400 µl of fibroblast medium and add to respective wells; to the third well add 400 µl fibroblast medium. Add 1 mL of medium the next day.
9. One or two days later transfer the cells into a 6-well plate and grow until 60-70% confluence.
10. Supplement the fibroblast growth medium with hygromycin B (400 µg/mL) and add 2 mL to each well.
11. Keep the cells under selection until all cells in the control well are dead (up to 12 days), changing media every 2-3 days.
12. Freeze vials of fibroblasts after selection. Label as FM followed by the cell identification number/name.

3. Transdifferentiation protocol

1. Seed transduced FM onto 10 cm² dishes with 30-40% confluence. In a 12-well plate, seed 6 x 10⁴ cells (this is dependent on the individual cell line).
   NOTE: For immunostaining, seed cells onto glass coverslips or chamber slides coated with Matrigel. Dilute Matrigel at 1:10 in DMEM medium, add a volume enough to cover the surface, and let the slides sit at room temperature for one hour. Aspirate off right before seeding the cells.
2. For myoblasts induction, when the fibroblasts reached 70% confluence (Figure 3A), rinse the cell surface with PBS and add fresh myoblast media supplemented with fresh 8 µg/mL doxycycline.
   NOTE: The success of differentiation is compromised past 80% confluence.
3. After two to three days later, cells are 90-95% confluent and their morphology will have changed (Figure 3B). Rinse the cell surface with PBS and add fresh differentiation media supplemented with fresh 8 µg/mL doxycycline.
4. Continue to change media every 2-3 days without passaging until myotubes are established (confirm via morphology) (Figure 3C).
5. Seven to ten days after starting myotube differentiation, cells should be fully differentiated and may start to detach or die. Before this happens, harvest myotubes for further analysis.
   NOTE: The time course of myotube formation depends on the cell line. Mutations in muscle-related proteins may interfere in the myogenic potential. When myotubes start to appear bright and look white at the borders it is a signal they are starting to detach (Figure 4).
6. To harvest myotubes, collect media and transfer it to a 50 mL conical tube. The medium may contain myotubes that have detached.
7. Rinse the myotubes with 5 mL PBS and transfer PBS to the 50 mL tube.
8. Add 3 mL of 0.25 % trypsin to the cell surface. Place the dish in the incubator for 5 min. Check the dish under the microscope to see if the cells are lifted. If not, place it back in the incubator for an additional 5 min.
9. Once the cells are detached, add 7 mL of fibroblast media to the dish and pipette up and down to resuspend the cells. Collect the cells to the 50 mL conical tube.
10. Centrifuge at 1,200 x g for 7 min at 4 °C.
11. Carefully aspirate off the liquid, without disturbing the pellet. Store the pellets at -80 °C until further processing.

4. Immunostaining of differentiated myotubes

NOTE: For immunostaining, grow the cells in glass coverslips or chamber slides as noted above.

1. Once myotubes are fully differentiated, aspirate off media and carefully rinse the slides with PBS. Aspirate PBS off.
2. Add fresh 4% PFA (500 µL per well of a 12-well plate) and incubate at room temperature for 10 min. Aspirate PFA off.
3. Rinse with 1 mL PBS.
4. Incubate with 0.2 M glycine at room temperature, for 10 min. Aspirate glycine off.
5. Permeabilize with PBS 0.5% TritonX-100 (300 µL/well of a 12-well plate), for 10 min with gentle agitation.
6. Block with 300 µL/well of blocking solution, for 10 min with gentle agitation.
7. Incubate with primary antibody diluted 1:50 in 300 µL of blocking solution, for 2 hours at room temperature, with gentle shaking.
8. Rinse three times with 1 mL/well of PBS for 5 min, with gentle shaking.
9. Incubate with secondary antibody diluted 1:500 in 300 µL of blocking solution, for 1 hour, at room temperature, with gentle shaking. Cover the plate with aluminum foil.
10. Rinse three times with 1 mL/well of PBS for 5 min, with gentle shaking.
11. Incubate with DAPI diluted in PBS for 10 minutes. Rinse three times with 1 mL/well of PBS.
12. Add a drop of mounting medium to a glass slide. Remove the coverslip with forceps and place it face down on the drop of mounting medium.
13. Invert slide onto a paper towel and gently press to remove bubbles and excess of mounting medium.
14. Seal the slides with nail polish and store at 4 °C until imaging.

5. Antisense oligonucleotide transfection

NOTE: The protocol below is for transfection of a 6-well plate. Adjust volumes accordingly for smaller or bigger plates. The transfection is done in 100% confluent myoblasts when the cells are ready for the differentiation step.

1. Aspirate the myoblast growth media and rinse the cells with 1 mL PBS.
2. Add 500 µL/well of OptiMEM media and incubate at 37 °C for 1 hour.
3. Dilute the antisense oligonucleotide (AON) in 100 µL of OptiMEM to the desired final concentration (i.e. 50 nM, 100 nM, 200 nM, 500 nM). Incubate at room temperature for 5 min.
   NOTE: This protocol is optimized for 2'omethyl-phosphorothioate AONs.
4. Mix the lipofectamine with OptiMEM (final volume of 100 µL) to give a final ratio of 1:1 (µg DNA: µL lipofectamine). Incubate at room temperature for 5 min.
5. Combine the diluted lipofectamine with the diluted AON. Mix gently by pipetting and incubate for 20 min at room temperature to allow complex formation. Avoid air bubbles.
6. Add 200 µL of lipofectamine and AON mix to respective wells. Incubate the cells overnight at 37 °C, 5% CO₂.
7. The following day remove the transfection mix and add 2 mL of warm differentiation media supplemented with doxycycline.
8. Collect cells at least three days later for RNA extraction or seven to 21 days in case of protein analysis.
   ​NOTE: The days of differentiation necessary to detect RNA and/or protein expression may vary accordingly to the gene of interest or the cell line. In the case of DMD, it's possible to detect its mRNA within three days. Dystrophin protein detection requires at least seven days. This will vary depending on the cell line. High concentrations of AON and transfection reagent can impact the transdifferentiation.

6. AAV1-U7 transduction

NOTE: This protocol was optimized for 6-well plates. Adjust the volumes proportionally to the culture surface area. The transduction is done in 100% confluent myoblasts when the cells are ready for the differentiation step. AAV1 is the AAV serotype with the best transduction capacity of cultured myoblasts.

1. Aspirate off the myoblast growth medium and rinse the cells with 1 mL PBS.
2. Dilute 0.5-1 x 10¹¹ viral particles of AAV1-U7 in 700 µL of warm differentiation media supplemented with doxycycline.
   ​NOTE: We use qPCR to determine the viral concentration. The amount of virus to be used may vary depending on the quantification method and should be determined previously using a reporter assay.
3. Add the viral mix to the well by dropping it homogenously.
4. The following day, add 1.3 mL of warm differentiation media supplemented with doxycycline.
5. Collect the cells at least three days later for RNA extraction or seven to 21 days in case of protein analysis.

7. RNA extraction

NOTE: All material used during this step should be RNase free.

1. Add 500 µL of TRIzol per pellet and pipet up and down several times to ensure that cells are homogenously lysed.
2. Transfer the cell lysate in a 1.5 mL tube and incubated for 5 min at room temperature.
3. Add 100 µL of chloroform and shake manually for 15 s. Incubate for 5 min at room temperature.
4. Centrifuge at 12,000 x g for 15 min at 4 °C. Collect the aqueous phase (upper one) and transfer it to a new 1.5 mL tube.
5. For 1 volume of the aqueous phase, add 1 volume of ethanol 100% and mix by pipetting.
   NOTE: We recommend column purification and concentration.
6. Transfer the sample to a Zymo-Spin IC column in a collection tube and centrifuge at 12,000 x g for 30 s. Discard the flow-through.
7. For in-column DNase I digestion, pre-wash the column with 400 µL RNA Wash Buffer. Centrifuge at 12,000 x g for 30 s. Discard the flow-through.
8. Prepare 40 µL of DNase reaction mix per sample. Mix 5 µL DNase I with 35 µL DNA Digestion Buffer.
9. Add the mix directly to the column matrix. Incubate at room temperature for 15 min.
10. Add 400 µL RNA Prep Buffer to the column and centrifuge at 12,000 x g for 30 s. Discard the flow-through.
11. Add 700 µL RNA Wash Buffer to the column and centrifuge at 12,000 x g for 30 s. Discard the flow-through.
12. Add 400 µL RNA Wash Buffer to the column and centrifuge at 12,000 x g for 2 min to ensure complete removal of the wash buffer. Transfer the column carefully into an RNase-free 1.5mL tube.
13. Add 15 µL nuclease-free water directly to the column matrix. Incubate for 5 min and centrifuge at 12,000 x g for 1 minute.
    NOTE: Collect the eluted RNA and apply it again to the column to increase yield. Centrifuge at 12,000 x g for 1 minute.
14. Place samples on ice and quantify the samples in a Nanodrop.
15. Store samples at -80 °C.

8. RT-PCR analysis

NOTE: In this step, we present a suggestion of reagents to detect the expression of dystrophin mRNA, but it can be easily adapted to other reagents of choice.

1. Reverse transcription
   1. Thaw all the reagents and keep them on ice.
   2. Prepare a mix with 4 µL of 5x Reaction Buffer, 2 µL of dNTP Mix (10 mM), 1 µL of RiboLock RNase Inhibitor, and 1 µL of RevertAid RT.
   3. Mix the tube gently and centrifuge briefly.
   4. In 0.2 mL PCR tubes, add the adequate volume of RNA in order to have 1 µg per reaction. Add nuclease-free water q.s.p 12 µL. Include one tube without the reverse transcriptase as a negative control and one tube with nuclease-free water instead of RNA.
   5. Distribute 8 µL of reaction mix per tube. The total volume is 20 µL.
   6. Place tubes in a thermocycler and incubate for 5 min at 25 °C followed by 60 min at 42 °C. Stop the reaction by heating at 70 °C for 5 minutes.
   7. Place the tubes on ice or at -20 °C for longer storage.
2. PCR
   NOTE: Design primers at exons junctions preferably.
   1. Vortex reagents and spin down before use.
   2. Prepare a master mix using 0.5 µL forward primer (25 µM), 0.5 µL reverse primer (25 µM), 12.5 µL 2x PCR Master Mix, and 8.5 µL of nuclease-free water per sample.
   3. Aliquot 22 µL of master mix into a tube for each sample.
   4. Add 3 µL of cDNA (150 ng) to its respective PCR tube. Add 3 µL of nuclease-free water to the PCR negative control tube.
   5. Vortex and spin down the PCR tubes.
   6. Incubate the tubes in a thermocycler at 95 °C for 3 min, 95 °C for 30 s, (Tm-5) °C for 30 s, 72 °C for (1 min/kb) 34 times, 72 °C for 5 min.
      NOTE: The optimal annealing temperature may be determined empirically. For the suggested master mix, subtract 5 °C from the primer melting temperature.
   7. Load 12 µL of the PCR reaction on an agarose gel and freeze the samples at -20 °C.

9. Detection of dystrophin expression by Western Blotting

NOTE: This protocol is optimized for dystrophin, a large membrane protein. Specific conditions may be needed for different proteins.

1. Protein extraction
   1. After 7-21 days of differentiation, collect cells with 5 mL of PBS with 100 µL 0.5 M EDTA, and 50 µL protease inhibitors. Incubate at 37 °C until cells detach. Centrifuge at 1,200 x g for 5 min at 4 °C. Snap freeze the pellet by dipping the tube in liquid nitrogen. Store the pellet at -80 °C or proceed to the lysis step.
   2. Prepare lysis buffer by adding 1% of digitonin, 1% protease inhibitor, 10% phosphatase inhibitor, and base buffer to total volume (60 µL per cell pellet).
   3. Add 60 µL of lysis buffer to the cell pellet, on ice. Sonicate for 5 s. Let sit on ice for 8 s. Repeat sonication and rest steps twice.
   4. Incubate samples on ice for 30 min.
   5. Centrifuge at 14,000 x g for 20 min at 4 °C.
   6. Transfer the supernatant to clean tubes.
   7. Quantify samples by bicinchoninic acid (BCA) assay, following manufacturer instructions.
   8. Mix the protein solution with the appropriate volume of Laemmli buffer. Make aliquots of 100 µg. If necessary, adjust the volume to 25 µl with base lysis buffer. Store samples at -80 °C.
2. Western blotting
   1. Thaw samples on ice.
   2. Denature the samples at 100 °C for 5 min, then cool them down in ice, spin down.
   3. Dilute the 20X Tris-acetate SDS running buffer in 200 mL dH₂O and add 500 µL antioxidant.
   4. Prepare the 3-8% Tris-acetate polyacrylamide gel by removing the comb and rinsing with dH₂O. Assemble the gel in the electrophoresis apparatus. Fill the inner chamber with running buffer.
   5. Load 5 µL of protein ladder and 25 µL of sample in the gel. Fill the outer chamber with running buffer.
   6. Run at 80 V for 1 h at 4 °C. Then, at 120 V for 2 h at 4 °C.
   7. Prepare 3 L of 1X transfer buffer with 150 mL of 20X methanol, 150 mL of 20X transfer buffer, and 2,700 mL of dH₂O. Cool it down to 4 °C.
   8. Cut 4 pieces of filter paper and one piece of nitrocellulose membrane. Soak the paper filter and membrane in a tray with transfer buffer.
   9. Gently remove the gel from the case and assemble it in the transfer apparatus with filter paper, membrane and sponges. The gel is placed on the negative side and the membrane on the positive side.
   10. Run transfer at 300 mA, stirring, at 4 °C, overnight.
   11. Block the membrane in 10 mL of blocking buffer for 1 h with gentle agitation, at room temperature.
   12. Prepare primary antibody solution with 10 mL of blocking buffer and 50 µL of dystrophin antibody (1:200).
   13. Discard the blocking buffer and add the primary antibody solution. Incubate with gentle agitation for at least 2 h at room temperature or overnight at 4 °C.
   14. Rinse the membrane three times with 0.1% Tween PBS, for 5 min with gentle agitation.
   15. Prepare secondary antibody solution using 10 mL of blocking solution, 2 µL of anti-rabbit antibody (1:5000), and 20 µL of 0.2% Tween.
   16. Add the secondary antibody solution to the membrane. Incubate for 1 h with gentle agitation, covered with aluminum foil to protect from light.
   17. Discard the antibody solution and rinse the membrane 3 times with 0.1% Tween PBS, for 5 min with gentle agitation, protected from light.
   18. Exposure and image the membrane on an imaging device.
   19. Stain the membrane for total protein with Revert 700 Total Protein stain, following manufacturer instructions.
       NOTE: Dystrophin detection by western blotting depends on the age/mutation of the patient and the cell's ability to fuse and stay attached enough time to accumulate enough dystrophin.

Results

This protocol shows how to establish human skin-derived fibroblast cultures and convert them into myoblasts and then into differentiated myotubes. This type of cell line is extremely useful for the study of neuromuscular disorders and in vitro testing of potential therapies.

A schematic representation of the fibroblast conversion is shown in Figure 1. Figure 2A shows a fragment of skin and the fibroblasts emerging from it. Th...

Discussion

To obtain FM cell lines with good quality, some steps are critical. The sooner the skin biopsy is processed, the greater the chances are to obtain healthy fibroblasts. The passage number of fibroblasts cultures is also important. Primary cells have limited proliferative capacity and after many passages, they enter in replicative senescence. Thus, it is better to have a stock of fibroblasts at a low passage number and transform cells at the earliest passage as possible.

Another important step ...

Materials

Name	Company	Catalog Number	Comments
100 mm dish	Corning	430167
0.25% Trypsin-EDTA, phenol red	Thermo Fisher	2500056
10X Phosphate buffered saline (PBS)	Fisher Scientific	BP3994
12-well plate	Corning	3513
20X Transfer buffer	Thermo Fisher	NP00061
20X Tris-acetate SDS running buffer	Thermo Fisher	LA0041
3-8% Tris-Acetate gel	Thermo Fisher	EA0378BOX
75 cm2 flask	Corning	430641U
Antibiotic-Antimicotic 100X	Thermo Fisher	15240062
Anti-myosin heavy chain, sarcomere antibody	Developmental Studies Hybridome Bank	MF20 supernatant	Dilution 1:50
Antioxidant	Thermo Fisher	NP0005
BCA Protein Assay	Thermo Fisher	23227
Chloroform	Sigma-Aldrich	C2432
DAPI	Thermo Fisher	D3571	Dilution 1:1000
Digitonin	Millipore Sigma	300410250MG
Dimethyl sulfoxide	Sigma-Aldrich	D2438
DMEM, High glucose, GlutaMAX supplement, Pyruvate	Thermo Fisher	10569044
DNAse I set (250U)	Zymo Research Corporation	E1010
Doxycycline Hydrochloride	Fisher Scientific	BP2653-5
Dup2 human primers	Fw_5' GCTGCTGAAGTTTGTTGG TTTCTC 3'	Rv_5' CTTTTGGCAGTTTTTGCC CTGT 3'
Dystrophin antibody	Abcam	ab15277	Dilution 1:200
Fetal bovine serum	Thermo Fisher	16000
Glycine	Sigma-Aldrich	G8898
Goat anti-mouse, Alexa Fluor 488	Thermo Fisher	A11001	Dilution 1:1000
Halt Protease inhibitor cocktail 100X	Thermo Fisher	78430
Hemocytometer	Hausser Scientific	3100
Hygromycin B	Thermo Fisher	10687010
IRDye 680RD goat anti-Rabbit IgG (H+L)	Li-Cor	926-68071	Dilution 1:5000
Lab-Tek II CC2 chamber slide system	Thermo Fisher	15852
Laemmli	Bioworld	105700201
Lipofectamine 3000 Transfection Reagent	Thermo Fisher	L3000008
Matrigel GFR membrane matrix	Corning	354230
Methanol	Fisher Scientific	A412P-4
Mr. Frosty Freezing Container	Thermo Fisher	51000001
Nitrocellulose membrane 0.45 µm	GE Healthcare Life Sciences	10600002
Normal Goat serum control	Thermo Fisher	10000C
Odyssey Blocking Buffer (PBS)	Li-Cor	927-40003	Blocking buffer for Western blot
Opti-MEM I Reduced Serum Medium	Thermo Fisher	11058021
Paraformaldehyde	Sigma-Aldrich	158127
PCR master mix	Thermo Fisher	K0172
Phosphatase inhibitor	Thermo Fisher	A32957
Precision Plus Protein Dual Color Standards	Bio Rad	1610374
Puromycin	Thermo Fisher	A1113803
Revert 700 Total Protein Stain for Western Blot Normalization	Li-Cor	926-11021
RevertAid kit	Thermo Fisher	K1691
RNA Clean & Concentrator-25	Zymo Research Corporation	R1018
Scalpels	Aspen Surgical	372611
Skeletal Muscle Cell Differentiation medium	Promocell	C23061
Skeletal Muscle Cell Growth medium	Promocell	C23060
Triton X-100	Acros Organics	215682500
TRIzol reagent	Thermo Fisher	15596026
Tween 20	Fisher Scientific	BP337500
Ultra low temperature freezer	Thermo Scientific	7402
UltraPure 0.5M EDTA, pH 8.0	Thermo Fisher	15575020
Vectashield antifade mounting medium	Vector Labs	H1000