Optimization of Processing of Tiebangchui with Highland Barley Wine Based on the Box-Behnken Design Combined with the Entropy Method

Summary

The present protocol describes an efficient method for optimization of the processing technology of Tiebangchui processed with highland barley wine based on a Box-Behnken design response surface combined with the entropy method.

Abstract

The processing of toxic ethnomedicines is of great significance for their safe clinical application. Thus, the limitations of traditional processing should be addressed, and the processing method of ethnomedicines should be standardized using modern research methods. In this study, the processing technology of a commonly used Tibetan medicine Tiebangchui (TBC), the dried root of Aconitum pendulum Busch, processed with highland barley wine was optimized. Diester-diterpenoid alkaloid (DDA) (aconitine, 3-deoxyaconitine, 3-acetylaconitine) and monoester-diterpenoid alkaloid (MDA) (benzoylaconine) content were used as evaluation indicators, and the weight coefficient of each evaluation index was determined by the entropy method.

The single factor test and Box-Behnken design were used in investigating the influence of the ratio between highland barley wine and TBC, slice thickness of TBC, and processing time. Comprehensive scoring was performed according to the objective weight of each index determined by the entropy method. The optimal processing conditions of TBC with highland barley wine were as follows: the amount of highland barley wine is five times that of TBC, a soaking time of 24 h, and a TBC thickness of 1.5 cm. The results showed that the relative standard deviation between the verification test and predicted value was less than 2.55% and the optimized processing technology of TBC processed with highland barley wine is simple, feasible, and stable, and so can provide a reference for industrial production.

Introduction

Tiebangchui (TBC), the dried root of Aconitum pendulum Busch, is a well-known Tibetan medicine and was initially recorded in the classic Tibetan medical book "Four Medical Tantra"^1,2. According to "Drug Standards of the Ministry of Health of the People's Republic of China (Tibetan Medicine)", TBC is effective in expelling cold, relieving pain, dispelling wind, and calming shock, and is commonly used to treat rheumatoid arthritis in clinics^3,4,5.

TBC mainly conta....

Protocol

In this study, the processing technology of TBC processed with highland barley wine was optimized with a Box-Behnken design combined with the entropy method. DDA and MDA content were used as evaluation indicators, and the weight coefficient of each evaluation index was determined by the entropy method.

1. Experimental preparation

1. Prepare highland barley wine¹⁵.
   1. Take 500.00 g of black highland barley rice and add five times the amount of water. Cook the rice until the remaining water is absorbed (~2 h). Pour it out, wait until the temperature falls to 37 °C, add 4 g of Jiu....

Results

In this study, the precision, stability, repeatability, and sample recovery of TBC indicated that the method is feasible. The four index components in TBC had a good linear relationship within a specific concentration range. Typical chromatograms are shown in Figure 1. The precision test results (Table 8) showed that the relative standard deviation (RSD) of the peak areas were 2.56%, 1.49%, and 2.03% for benzoylaconine, aconitine, and 3-deoxyaconitine, respectively, and 0.21.......

Discussion

As a commonly used Tibetan medicine with toxic effects, the toxicity-attenuating effect of processing is extremely important for TBC's clinical application²⁵. In this study, the processing technology of TBC processed with highland barley wine was optimized. By reviewing the main active ingredients and relating the pharmacological effects of TBC, we found that TBC alkaloids have anti-inflammatory and analgesic effects and can be used to treat rheumatoid arthritis. In this study, a moderate toxi.......

Materials

Name	Company	Catalog Number	Comments
Aconitine	Chengdu Push Biotechnology Co.,Ltd	PS000905
3-Acetylaconitine	Chengdu Push Biotechnology Co.,Ltd	PS010552
3-Deoxyaconitine	Chengdu Push Biotechnology Co.,Ltd	PS011258
Benzoylaconine	Chengdu Push Biotechnology Co.,Ltd	PS010300
Circulating water vacuum pump	Gongyi City Yuhua Instrument Co., Ltd	SHZ-DIII
Design-Expert	State-East Corporation	8.0.6
Electric constant temperature drying oven	Shanghai Yuejin Medical Equipment Co., Ltd	101-3-BS
Electronic analytical balance	Shanghai Liangping Instruments Co., Ltd.	FA1004
High performance liquid chromatography	Shimadzu Enterprise Management (China) Co., Ltd	shimadzu 2030
Highland barley rice	Kangding City, Ganzi Tibetan Autonomous Prefecture, Sichuan Province	20221015
Millipore filter	Tianjin Jinteng Experimental Equipment Co., Ltd	φ13 0.22 Nylon66
Rotary evaporator	Shanghai Yarong Biochemical Instrument Factory	RE-2000A
Starter of liquor-making	Angel Yeast CO., Ltd	BJ22-104
Ultra pure water systemic	Merck Millipore Ltd.	Milli-Q
Ultrasonic cleansing machine	Ningbo Xinyi Ultrasonic Equipment Co., Ltd	SB-8200 DTS