Hematoxylin-Eosin Staining to Measure Colony Growth (Days 4-7)
6:06
Time Lapse Microscopy
7:12
Results: Analysis of Migration of Cells Sheets Using the Dot Assay
8:55
Conclusion
Transkript
The overall goal of the dot assay is to assess the migratory phenotype of attached cohesive cell sheets in response to microenvironmental cues. This method can help answer key questions in the cancer field, such as how cytokines or growth factors
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Cell migration is essential for development, tissue maintenance and repair, and tumorigenesis, and is regulated by growth factors, chemokines, and cytokines. This protocol describes the dot assay, a two-dimensional, unconstrained migration assay to assess the migratory phenotype of attached, cohesive cell sheets in response to microenvironmental cues.