Using Terminal Transferase-mediated dUTP Nick End-labelling (TUNEL) and Caspase 3/7 Assays to Measure Epidermal Cell Death in Frogs with Chytridiomycosis
Results: Quantification of Epidermal Cell Death in Frogs with Chytridiomycosis using TUNEL Assay and Caspase 3/7 Assay
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Conclusion
Transkript
The overall goal of this experiment is to observe the affect of amphibian fungal pathogen batrachochytrium dendrobatidis on apoptosis and cell death in the epidermal tissue of frogs. This method can help us understand wildlife disease such as if i
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We quantify epidermal cell death in frogs with chytridiomycosis using two methods. First, we use terminal transferase-mediated dUTP nick end-labelling (TUNEL) in situ histology to determine differences between clinically infected and uninfected animals. Second, we conduct a time series analysis of apoptosis over infection using a caspase 3/7 protein analysis.