Cell-Free Protein Synthesis Batch Format Reactions
6:25
Quantification of the Reporter Protein, [sfGFP]
7:24
Results: Modification and Optimization of the Cell-Free Platform Conditions
9:01
Conclusion
Transkript
This protocol simplifies and clarifies the methods for implementing self re-protein synthesis by non-experts. Improved access to these methods will help demarketize the platform and the broad set of applications that it enables. The main advantage
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This protocol details the steps, costs, and equipment necessary to generate E. coli-based cell extracts and implement in vitro protein synthesis reactions within 4 days or less. To leverage the flexible nature of this platform for broad applications, we discuss reaction conditions that can be adapted and optimized.