Setting Up and Incubating the ITO (Indium Tinoxide)-treated Slide for Cocultures
4:04
Drying Slide and Preparing for MALDI-TOF MS (Matrix-assisted Laser Desorption/Ionization Mass Spectrometry)
5:48
Results: Proper Sample Preparation and Representative IMS Data
7:16
Conclusion
Transkript
Our protocol can map small molecule chemical communication between cells and tissues, and we have specifically applied this to explore small molecules in ovarian cancer metathesis. The main advantage of our technique is the ability to measure smal
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A novel method of sample preparation was developed to accommodate cell and tissue coculture to detect small molecule exchange using imaging mass spectrometry.