Measuring ATPase Activities and Inhibitory Effects of Small Molecule Inhibitors
5:48
Analyzing Data
7:54
Results: Fluorescence Intensity
9:26
Conclusion
Transkript
This protocol is applicable to a wide range of screening projects aimed at developing ATPase inhibitors, either as probes for basic research or for potential clinical compounds. This method has been optimized for semi-high-throughput screening app
Bu içeriğe erişmek için giriş yapın veya ücretsiz denemenizi başlatın
A nicotinamide adenine dinucleotide (NADH)-coupled ATPase assay has been adapted to semihigh throughput screening of small molecule myosin inhibitors. This kinetic assay is run in a 384-well microplate format with total reaction volumes of only 20 µL per well. The platform should be applicable to virtually any ADP producing enzyme.