This method can answer key questions about the phosphorylation of the five-prime end of DNA or RNA molecules by a special class of enzymes known as polynucleotide kinase. The main advantage of this technique is it has the resolution to detect very
Bu içeriğe erişmek için giriş yapın veya ücretsiz denemenizi başlatın
This protocol describes a nonradioactive assay to measure kinase activity of polynucleotide kinases (PNKs) on small DNA and RNA substrates.