Labeled microscale thermophoresis obtains disassociation constants, or KDs, of diverse biochemical interactions efficiently. This protocol and the accompanied video give the approximate affinity for Vam7, the only soluble SNARE protein in yeast, t
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Microscale thermophoresis obtains binding constants quickly at low material cost. Either labeled or label free microscale thermophoresis is commercially available; however, label free thermophoresis is not capable of the diversity of interaction measurements that can be performed using fluorescent labels. We provide a protocol for labeled thermophoresis measurements.