Staining of Cell Suspension for Mononuclear Phagocytes
6:49
Results: Mononuclear Phagocyte Populations in the Mouse Eye
9:26
Conclusion
Transkript
This protocol is designed to digest whole mouse eyes into a single cell suspension for multi-parameter flow cytometry of mononuclear phagocytes which include macrophages, monocytes, microglia, and dendritic cells. The main advantage of this techni
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This protocol provides a method to digest whole eyes into a single cell suspension for the purpose of multi-parameter flow cytometric analysis in order to identify specific ocular mononuclear phagocytic populations, including monocytes, microglia, macrophages, and dendritic cells.