In Situ Transduction and Isolation of Bladder Mucosa
1:41
Mechanical Stimulation of Individual Urothelial Cells and Ca2+ Imaging
3:16
Data Analysis
4:03
Results: Analysis of Mechano-Activated Ca2+ Transients in Urothelial Cells
4:49
Conclusion
Transkript
This protocol describes a technique to study mechanically-evoked calcium event in an ex vivo urothelial preparation. The technique is relatively easy to execute and it could be adapted to study mechanically-evoked calcium event in other native tis
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This protocol describes a methodology to assess the function of mechanically activated ion channels in native urothelial cells using the fluorescent Ca2+ sensor GCaMP5G.