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Method for Measurement of Viral Fusion Kinetics at the Single Particle Level

DOI :

10.3791/1484-v

September 7th, 2009

September 7th, 2009

12,724 Views

1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 2Howard Hughes Medical Institute, Harvard Medical School

We present an in vitro, two-color fluorescence assay to visualize the fusion of single virus particles with a fluid target bilayer. By labeling viral particles with fluorophores that differentially stain the viral membrane and its interior, we are able to monitor the kinetics of hemifusion and pore formation.

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