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Mucociliary Epithelial Organoids from Xenopus Embryonic Cells: Generation, Culture and High-Resolution Live Imaging

DOI :

10.3791/61604-v

7:44 min

July 28th, 2020

July 28th, 2020

4,197 Views

1Center for Vascular Research, Institute for Basic Science

We describe a simple protocol to develop mucociliary epithelial organoids from deep ectoderm cells isolated from Xenopus laevis embryos. The multipotent progenitors regenerate epithelial goblet cell precursors and allow live tracking of the initiation and progression of the cell transitions on the surface of organoids.

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Mucociliary Epithelial Organoids

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