Demonstration of Self-Assembled Cell Sheet Culture and Manual Generation of a 3D Tendon/Ligament-Like Organoid by using Human Dermal Fibroblasts

1.1K Views

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03:35 min

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June 21st, 2024

DOI :

10.3791/66047-v

June 21st, 2024

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Ana Luísa Graça¹, Niklas Kroner-Weigl², Viviana Reyes Alcaraz¹, Sigrid Müller-Deubert¹, Maximilian Rudert³, Denitsa Docheva¹

¹Department of Musculoskeletal Tissue Regeneration, Orthopaedic Hospital König-Ludwig-Haus, University of Würzburg, ²Laboratory for Experimental Trauma Surgery, Department of Trauma Surgery, University Hospital Regensburg, ³Department of Orthopaedics, Orthopaedic Hospital König-Ludwig-Haus, University of Würzburg

Transcript

Summary

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Self assembled Cell Sheets

3D Tendon Organoid

Human Dermal Fibroblasts

Tissue Engineering

Regenerative Medicine

In Vitro Tenogenesis

Collagen Type I

Extracellular Matrix ECM

Tendon ligament Model

Differentiation Protocol

Dynamic Mechanical Stimulation

Cellular Architecture

Rod like Organoids

Expansion Stimulation Maturation

Chapters in this video

0:00

Introduction

0:51

2D Expansion, 2D Stimulation, and 3D Organoid Maturation of Adult Normal Human Dermal Fibroblasts for Tendon Research and Engineering

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