Investigating Angiogenesis on a Functional and Molecular Level by Leveraging the Scratch Wound Migration Assay and the Spheroid Sprouting Assay

985 Views

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09:16 min

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May 31st, 2024

DOI :

10.3791/66954-v

May 31st, 2024

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Julian Rapp*¹^,², Jan Ness*¹^,³, Paula Liang¹, Martin J. Hug⁴, Hansjürgen Agostini¹, Günther Schlunck¹, Clemens Lange⁵, Felicitas Bucher¹

¹Eye Center, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, ²Department of Medicine I, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, ³Institute of Pharmaceutical Sciences, Faculty of Chemistry and Pharmacy, University of Freiburg, ⁴Pharmacy, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, ⁵Ophtha-Lab, Department of Ophthalmology, St. Franziskus Hospital Muenster

Summary

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Angiogenesis

Scratch Wound Migration Assay

Spheroid Sprouting Assay

Molecular Mechanisms

In Vitro Assays

Endothelial Cells

Human Umbilical Vein Endothelial Cells HUVECs

Translational Research

Chapters in this video

0:00

Introduction

1:30

Scratch Wound Migration Assay of Human Umbilical Vein Endothelial Cells (HUVECs) for Investigating Angiogenesis In Vitro

3:13

Fixing and Blocking 2D Human Umbilical Vein Endothelial Cell (HUVEC) Cultures for Immunohistochemical Analysis

4:49

Spheroid Sprouting Assay of Human Umbilical Vein Endothelial Cells (HUVECs) for Investigating Angiogenesis In Vitro

7:41

Immunocytochemistry of Human Umbilical Vein Endothelial Cell (HUVEC) Spheroids in a 3D Collagen Matrix for Investigating Angiogenesis In Vitro

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