Visualization of Mitochondrial DNA Replication in Individual Cells by EdU Signal Amplification

22.9K Views

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09:36 min

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November 15th, 2010

DOI :

10.3791/2147-v

November 15th, 2010

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Kristine M. Haines¹, Eva L. Feldman², Stephen I. Lentz³

¹Michigan Research Community, Undergraduate Research Opportunity Program, University of Michigan, ²Department of Neurology, University of Michigan, ³Department of Internal Medicine, Division of Metabolism, Endocrinology, and Diabetes, University of Michigan

Summary

Chapters in this video

0:00

Title

0:58

Introduction

1:56

Preparation of Neurons

3:38

Click-iT 5-ethynyl-2-deoxyuridine (EdU) Labeling

5:31

Tyramide Signal Amplification (TSA) of EdU Signal

7:09

Visualization of Mitochondrial DNA Replication as a Marker for Mitochondiral Biogenesis

8:37

Conclusion

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