High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution
2.7K Views
•
10:47 min
•
May 5th, 2023
DOI :
May 5th, 2023
•Transcript
Chapters in this video
0:00
Introduction
1:21
Preparation of siRNA Mixture for Mitochondrial DNA Synthesis
2:00
Cell Transfection and 5‐Bromo‐2’‐Deoxyuridine (BrdU) Incorporation in HeLa Cells
3:14
5-Bromo-2'-Deoxyuridine (BrdU) Labeling of Mitochondrial DNA in HeLa Cells
4:51
HeLa Cell Fixation with Hoechst 33342 and Blocking
5:51
Addition of Primary and Secondary Antibodies to the Transfected HeLa Cells to Monitor the mtDNA Distribution
7:49
Quantitative Image Analysis to Measure the mtDNA and Gene Expression Levels in the Transfected HeLa Cells
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