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Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) for Analysis of Multiprotein Complexes from Cellular Lysates

130.2K Views

12:03 min

February 24th, 2011

DOI :

10.3791/2164-v

February 24th, 2011


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Blue Native Polyacrylamide Gel Electrophoresis

Chapters in this video

0:05

Title

1:21

Introduction

1:58

Preparation of Dialyzed Cell Lysate

4:48

Pouring of Blue Native-gels

7:04

Separation of Dialyzed Cell Lysate by Blue Native-PAGE

7:53

Second Dimension SDS-PAGE

9:25

Representative Results for Blue Native-PAGE Analysis of Multiprotein Complexes from Cellular Lysates

11:18

Conclusion

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