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Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization

DOI :

10.3791/57758-v

June 1st, 2018

June 1st, 2018

9,237 Views

1Department of Microbial Pathogenesis, Yale University School of Medicine, 2Program in Vertebrate Developmental Biology, Departments of Pediatrics and Genetics, Yale University School of Medicine, 3Department of Internal Medicine, Yale University School of Medicine

Here, we present a protocol to spatially and temporally assess the presence of viable microbiota in tick guts using a modified whole-mount in situ hybridization approach.

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