Quantitative Assessment of Immune Cells in the Injured Spinal Cord Tissue by Flow Cytometry: a Novel Use for a Cell Purification Method
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08:00 min
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April 9th, 2011
DOI :
April 9th, 2011
•1Institute for Memory Impairments and Neurological Disorders, University of California, 2Physical Medicine & Rehabilitation, University of California, 3Anatomy & Neurobiology, University of California, 4Sue and Bill Gross Stem Cell Research Center, University of California, 5Section of Molecular Biology, University of California, 6Reeve-Irvine Research Center, University of California
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Chapters in this video
0:05
Title
1:23
Dissociation of Spinal Cord Tissue
2:47
Creating OptiPrep Gradient Solutions and Separating Lipid/Myelin Debris from Cells
4:27
Immunolabeling of Specific Immune Cells and Flow Cytometric Quantitative Assessment
6:16
Removal of Lipid/Myelin Debris Enhances Quantitative Detection of Cellular Inflammation in the Injured Spinal Cord Tissue by Flow Cytometry
7:33
Conclusion
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