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JoVE Journal

Immunology and Infection

This content is Open Access.

Quantitative Assessment of Immune Cells in the Injured Spinal Cord Tissue by Flow Cytometry: a Novel Use for a Cell Purification Method

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08:00 min

April 9th, 2011

April 9th, 2011

21,977 Views

0:05

Title

1:23

Dissociation of Spinal Cord Tissue

2:47

Creating OptiPrep Gradient Solutions and Separating Lipid/Myelin Debris from Cells

4:27

Immunolabeling of Specific Immune Cells and Flow Cytometric Quantitative Assessment

6:16

Removal of Lipid/Myelin Debris Enhances Quantitative Detection of Cellular Inflammation in the Injured Spinal Cord Tissue by Flow Cytometry

7:33

Conclusion

Quantification of cellular inflammation in the injured/pathological CNS by flow cytometry is complicated by lipid/myelin debris that can have similar size and granulation to cells, decreasing sensitivity/accuracy. We have advanced a cell preparation method to remove myelin debris and improve cell detection by flow cytometry in the injured spinal cord.

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