JoVE Logo
Faculty Resource Center

Sign In

Seven Steps to Stellate Cells

DOI :

10.3791/2710-v

May 10th, 2011

May 10th, 2011

32,816 Views

1Immune Disease Institute, Program in Cellular and Molecular Medicine at Children's Hospital, Department of Pathology, Harvard Medical School

Here we describe a method for the isolation of hepatic stellate cells from mouse liver. For stellate cell purification, mouse livers are digested in situ and in vitro by pronase-collagenase treatment prior to density gradient centrifugation. This technique yields highly pure hepatic stellate cells.

-- Views

Related Videos

article

Isolation of Mouse Peritoneal Cavity Cells

article

Murine Model of CD40-activation of B cells

article

Measuring Calpain Activity in Fixed and Living Cells by Flow Cytometry

article

Induction and Assessment of Class Switch Recombination in Purified Murine B Cells

article

Assessing Stomatal Response to Live Bacterial Cells using Whole Leaf Imaging

article

Colony Forming Cell (CFC) Assay for Human Hematopoietic Cells

article

Antigen Specific In Vivo Killing Assay using CFSE Labeled Target Cells

article

Retroviral Transduction of T-cell Receptors in Mouse T-cells

article

Isolation of Brain and Spinal Cord Mononuclear Cells Using Percoll Gradients

article

Monitoring Immune Cells Trafficking Fluorescent Prion Rods Hours after Intraperitoneal Infection

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved