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Listeria monocytogenes Infection of the Brain

DOI :

10.3791/58723-v

October 2nd, 2018

October 2nd, 2018

12,031 Views

1Department of Microbiology and Immunobiology, Harvard Medical School

During infection, Listeria monocytogenes is capable of crossing the blood-brain barrier to colonize the brain. In this protocol, we demonstrate how to assess bacterial colonization of organs following infection of mice. A procedure to perform whole organ perfusion for specific determination of bacterial numbers in the brain parenchyma is provided.

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