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Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCR

DOI :

10.3791/58596-v

10:55 min

November 10th, 2018

November 10th, 2018

25,611 Views

1Institute of Veterinary Bacteriology Vetsuisse Faculty, University of Bern, 2Department of Veterinary Microbiology and Immunology and Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University

This protocol diagnoses Tilapia Lake Virus (TiLV) in tilapia tissues using RT-PCR methodologies. The entire method is described from tissue dissection to total RNA extraction, followed by cDNA synthesis and detection of TiLV by either conventional PCR or quantitative PCR using dsDNA binding a fluorescent binding dye.

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Tilapia Lake Virus

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