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Two Types of Assays for Detecting Frog Sperm Chemoattraction

DOI :

10.3791/3407-v

December 27th, 2011

December 27th, 2011

18,800 Views

1Department of Animal Sciences, University of Illinois, Urbana-Champaign, 2School of Life Sciences, Arizona State University

Eggs and the extracellular coatings around eggs frequently release peptides, proteins and small molecules that communicate with sperm to guide them to the egg thereby promoting fertilization. Using frog sperm we describe and compare two classes of assays used to detect sperm chemoattraction – sperm accumulation assays and sperm tracking assays.

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