Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration
10.7K Views
•
09:33 min
•
October 3rd, 2019
DOI :
October 3rd, 2019
•Sandra Guadalupe Gonzalez Malagon*1,2, Lisa Dobson*1,3, Anna M Lopez Muñoz1, Marcus Dawson1, William Barrell1,3, Petros Marangos2,4, Matthias Krause3, Karen J Liu1
1Centre for Craniofacial and Regenerative Biology, King's College London, 2Institute of Molecular Biology and Biotechnology, FORTH, Department of Biomedical Research, University of Ioannina, 3Randall Centre of Cell & Molecular Biophysics, King's College London, 4Department of Biological Applications and Technology, University of Ioannina
Chapters in this video
0:04
Title
1:05
Early Somite Stage Embryo Dissection (Day 1)
3:12
Live Cell Imaging of Murine Cranial Neural Crest Cells (Day 2)
4:36
Neural Crest Single Cell Tracking
5:22
Quantification of Neural Crest Cell Migratory Capacity
6:04
Quantification of Neural Crest Cell Area and Circularity
7:00
Results: Representative Cranial Neural Crest Cell Migration and Cell Shape Dynamics Analyses
8:43
Conclusion
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