Isolation of PBMCs and In Vitro Expansion Using an HBV Peptide Matrix
3:55
Analysis of HBV-specific CD4 T-Cell Responses by Intracellular Flow Cytometry
5:54
Identification of HBV-specific HLA-DR Restricted CD4 T-Cell Epitopes
8:07
Results: Identification of HBV-specific HLA-DR Restricted CD4 T-Cell Epitopes
10:06
Conclusion
Transcript
In a Chronic HBV infection, CD4 T cells play important roles in both viral clearance and the returners. This method enables us to evaluate HBV specific CD4 T cell responses and identify HBV specific CD4 T cell epitopes, simultaneously. Demonstrati
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Based on a hepatitis B virus (HBV)-derived peptide matrix, HBV-specific CD4 T-cell responses could be evaluated in parallel with identification of HBV-specific CD4 T-cell epitopes.