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Isolation of Basal Cells and Submucosal Gland Duct Cells from Mouse Trachea

13.3K Views

11:52 min

September 14th, 2012

September 14th, 2012

13,301 Views

0:05

Title

1:45

Dissection of Trachea

2:46

Cleaning and Cutting the Trachea

3:45

Enzyme Digestion and Processing into Single Cell Suspension

6:42

Staining for FACS and Sorting

7:23

Processing Sorted Cells for in vitro Culture and in vivo Model

9:40

Representative Data

11:19

Conclusion

Here we demonstrate our protocol for isolation of basal and submucosal gland duct cells from mouse tracheas. We also demonstrate the method of injecting stem cells into the dorsal mouse fat pad to create an in vivo model of submucosal gland regeneration.

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