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Isolation and Enrichment of Human Lung Epithelial Progenitor Cells for Organoid Culture

DOI :

10.3791/61541-v

July 21st, 2020

July 21st, 2020

7,235 Views

1Lung and Regenerative Medicine Institutes, Department of Medicine, Cedars-Sinai Medical Center

This article provides a detailed methodology for tissue dissociation and cellular fractionation approaches allowing enrichment of viable epithelial cells from proximal and distal regions of the human lung. Herein these approaches are applied for the functional analysis of lung epithelial progenitor cells through the use of 3D organoids culture models.

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Keywords 3D Organoid Culture

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