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Ex vivo Method for High Resolution Imaging of Cilia Motility in Rodent Airway Epithelia

DOI :

10.3791/50343-v

August 8th, 2013

August 8th, 2013

20,176 Views

1Department of Developmental Biology, University of Pittsburgh

An easy and reliable technique for visualizing and quantifying airway cilia motility and cilia generated flow using mouse trachea is described. This technique can be modified to determine how a wide range of factors influence cilia motility, including pharmacological agents, genetic factors, environmental exposures, and/or mechanical factors such as mucus load.

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