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Isolation of Murine Intestinal Mesenchyme Resulting in a High Yield of Telocytes

DOI :

10.3791/64169-v

6:35 min

March 24th, 2023

March 24th, 2023

1,032 Views

1Department of Developmental Biology and Cancer Research, The Institute for Medical Research Israel-Canada, The Hebrew University - Hadassah Medical School, 2Shanghai Institute of Immunology, Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine

Here, we present a protocol to isolate murine intestinal mesenchyme, including telocytes. These can be used for several applications, such as co-culture with mouse or human-derived organoids, to support growth and better reflect the situation in the original tissue.

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Isolation

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