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Chromatin Immunoprecipitation (ChIP) using Drosophila tissue

DOI :

10.3791/3745-v

March 23rd, 2012

March 23rd, 2012

24,840 Views

1Department of Biology, Johns Hopkins University

Recently high-throughput sequencing technology has greatly increased sensitivity of Chromatin Immunoprecipitation (ChIP) experiment and prompted its application using purified cells or dissected tissue. Here we delineate a method to use ChIP technique with Drosophila tissue, which can address the endogenous chromatin state in a well-characterized biological system.

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Chromatin Immunoprecipitation

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