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Transfection, Selection, and Colony-picking of Human Induced Pluripotent Stem Cells TALEN-targeted with a GFP Gene into the AAVS1 Safe Harbor

DOI :

10.3791/52504-v

February 1st, 2015

February 1st, 2015

18,910 Views

1National Institute of Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, 2Q Therapeutics

TALEN-mediated gene editing at the safe harbor AAVS1 locus enables high-efficiency transgene addition in human iPSCs. This protocol describes the procedures for preparing iPSCs for TALEN and donor vector delivery, transfecting iPSCs, and selecting and isolating iPSC clones to achieve targeted integration of a GFP gene to generate reporter lines.

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Transfection

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