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Preparation of Mouse Embryonic Fibroblast Cells Suitable for Culturing Human Embryonic and Induced Pluripotent Stem Cells

90.7K Views

09:38 min

June 21st, 2012

DOI :

10.3791/3854-v

June 21st, 2012


Transcript

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Mouse Embryonic Fibroblast Cells

Chapters in this video

0:05

Title

1:55

Isolation of Mouse Embryonic Fibroblasts

4:05

Inactivation and Plating MEFs (Feeder Cells Preparation)

5:14

Conditioned Medium Preparation and Measurement of Activin A using ELISA

7:41

Representative Conditioned Medium Preparation Results

9:08

Conclusion

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