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Expression, Purification, and Liposome Binding of Budding Yeast SNX-BAR Heterodimers

7.6K Views

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10:28 min

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December 6th, 2019

DOI :

10.3791/60413-v

December 6th, 2019

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7,608 Views

Mengxiao Ma¹, Shreya Goyal², Christopher G. Burd¹, Richard J. Chi²

¹Department of Cell Biology, Yale School of Medicine, ²Department of Biological Sciences, University of North Carolina at Charlotte

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Summary

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Membrane Remodeling

Liposome Binding

Yeast Expression

Protein Purification

IgG Sepharose Beads

Biophysical Properties

Lipid Specificity

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