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Isolation of Cardiomyocyte Nuclei from Post-mortem Tissue

DOI :

10.3791/4205-v

July 10th, 2012

July 10th, 2012

16,608 Views

1Strategic Research Center for Stem Cell Biology and Cell Therapy, University of Lund, 2Department of Cardiology Lund University Hospital, University of Lund

Cardiac nuclei are isolated via density sedimentation and immunolabeled with antibodies against pericentriolar material 1 (PCM-1) to identify and sort cardiomyocyte nuclei by flow cytometry.

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Isolation

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