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Isolation and Functional Characterization of Human Ventricular Cardiomyocytes from Fresh Surgical Samples

DOI :

10.3791/51116-v

April 21st, 2014

April 21st, 2014

16,952 Views

1Department NeuroFarBa, Division of Pharmacology, University of Florence, 2Department of Clinical and Experimental Medicine, Division of Physiology, University of Florence

Current knowledge on the cellular basis of cardiac diseases mostly relies on studies on animal models. Here we describe and validate a novel method to obtain single viable cardiomyocytes from small surgical samples of human ventricular myocardium. Human ventricular myocytes can be used for electrophysiological studies and drug testing.

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Isolation

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