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Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols

DOI :

10.3791/50075-v

January 2nd, 2013

January 2nd, 2013

17,853 Views

1Centre for Blood Research, University of British Columbia , 2Department of Pathology and Laboratory Medicine, University of British Columbia , 3Canadian Blood Services, University of British Columbia , 4Department of Chemistry, Life Sciences Centre, University of British Columbia

The cell membrane modification of red blood cells (RBCs) with hyperbranched polyglycerol (HPG) is presented. Modified RBCs were characterized by aqueous two phase partitioning, osmotic fragility and complement mediated lysis. The camouflage of surface proteins and antigens was evaluated using the flow cytometry and Micro Typing System (MTS) blood phenotyping cards.

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