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Measurement of Vacuolar and Cytosolic pH In Vivo in Yeast Cell Suspensions

DOI :

10.3791/50261-v

April 19th, 2013

April 19th, 2013

19,332 Views

1Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University

Vacuolar and cytosolic pH can be measured in live yeast (S. cerevisiae) cells using ratiometric fluorescent dyes localized to specific cellular compartments. We describe procedures for measuring vacuolar pH with BCECF-AM, which localizes to the vacuole in yeast, and cytosolic pH with a cytosolic ratiometric pH-sensitive GFP (yeast pHluorin).

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