Sign In

A subscription to JoVE is required to view this content.

Naïve Adult Stem Cells Isolation from Primary Human Fibroblast Cultures

14.0K Views

07:39 min

May 3rd, 2013

May 3rd, 2013

14,006 Views

0:05

Title

1:27

SKP Isolation from Primary Fibroblast Cultures

2:11

Sphere Culture Conditions

2:57

Immunocytochemistry for Stem Cell Markers

3:59

Realtime PCR Analysis of Expression Levels of Stem Cell Markers

5:01

Directed Differentiation into Smooth Muscle Cells

5:54

Results: SKP Spheres from Primary Fibroblast Cultures

7:16

Conclusion

We report a method to isolate naïve multipotent skin-derived precursor (SKP) cells from primary human fibroblast cultures. We show that these SKPs derived from fibroblast cultures share similar stem cell properties to the ones derived directly from human skin biopsies. These cells express the neural crest marker, nestin, in addition to the multipotent markers such as OCT4 and Nanog.

We use cookies to enhance your experience on our website.

By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.

Learn More