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The Specification of Telencephalic Glutamatergic Neurons from Human Pluripotent Stem Cells

DOI :

10.3791/50321-v

April 14th, 2013

April 14th, 2013

10,708 Views

1Department of Neuroscience, The University of Connecticut Health Center, 2Department of Genetics and Developmental Biology, The University of Connecticut Health Center, 3Stem Cell Institute, The University of Connecticut Health Center

This procedure yields telencephalic neurons by going through checkpoints which are similar to those observed during human development. The cells are allowed to spontaneously differentiate, are exposed to factors which push them towards the neural lineage, are isolated, and are plated onto coverslips to allow for terminal differentiation and maturation.

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